Table 1.
Number and characteristics of contigs and scaffolds for each of the five assemblies
| PacBio (Pb) | PacBio BioNano (PbBn) | PacBio Dovetail (PbDt) | PacBio BioNano Dovetail (PbBnDt) | PacBio Dovetail BioNano (PbDtBn) | |
|---|---|---|---|---|---|
| Assembly software | FALCON | FALCON Irys | FALCON HiRise | FALCON Irys HiRise | FALCON HiRise Irys |
| Contigs | 1, 073 | 1, 073 | 1, 121 | 1, 125 | 1, 121 |
| Contig Length | 396,973,838 | 396,973,942 | 396,973,838 | 396,973,942 | 396,973,934 |
| Contig N50a | 3,768,504 | 3,768,512 | 3,768,504 | 3,768,512 | 3,768,504 |
| Scaffolds | 1, 073 | 993 | 1, 005 | 965 | 942 |
| Scaffold Length | 396,973,838 | 401,421,527 | 396,985,438 | 401,429,527 | 399,955,467 |
| Maximum Scaffold Length | 13,488,151 | 22,885,216 | 19,275,758 | 12,137,306 | 12,557,854 |
| Scaffold N50a | 3,768,504 | 6,819,834 | 6,895,511 | 12,137,306 | 12,557,854 |
aN50 s were also adjusted to use an assembly length of 400 Mb for all assemblies in order to facilitate comparisons across assemblies. Scaffold and contig N50 s adjusted for a 400 Mb assembly size were identical to unadjusted N50 s shown above, except for the PbDt scaffold N50 for which the adjusted N50 was 6,348,449 nt