Table 1:

Summary of results of genetic distance-based evaluations of the AusPreda_12S mini-barcode

	FULL (1% threshold)				UNIQUE (3.5% threshold)
	Correct/true	Incorrect/false	Ambiguous	No ID	Correct/true	Incorrect/false	Ambiguous	No ID
Nearest neighbour	155	19	–	–	44	0	–	–
Best close match	147	3	0	24	42	0	0	2
Thresh ID	142	3	5	24	42	0	0	2

	FULL (3.5% threshold)
	Correct/true	Incorrect/false	Ambiguous	No ID
Best close match	152	6	0	16
Thresh ID	141	5	12	16

Summary of results of genetic distance-based evaluations of the AusPreda_12S mini-barcode conducted using the R package SPIDER to analyse the “FULL” (at 1% and 3.5% thresholds) and “UNIQUE” (at 3.5% threshold) reference sequence databases. The thresholds were calculated based on the minimum cumulative error (Additional file 6), and the 3.5% threshold for the “FULL” database allows for comparison between the 2 databases. The specified genetic distance thresholds were used for the BestCloseMatch and ThreshID analyses.