Table 3:
PCR and DNA sequencing results from 57 known-origin scat samples screened using the AusPreda_12S mini-barcode
| Sample | Scientific name | Common name | Amplified | Sequenced | Closest sequence match using BLAST | % IDa | e valueb |
|---|---|---|---|---|---|---|---|
| 100111–27 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 1.55E-84 |
| 120111–02 | Canis lupus familiaris | Dog | Y | Y | Dog | 100 | 6.52E-78 |
| 121010–11 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 1.22E-85 |
| 121010–16 | Canis lupus familiaris | Dog | Y | Y | Dog | 98.4 | 2.08E-83 |
| 121010–17 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 1.98E-83 |
| 121010–30 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 5.54E-84 |
| 121010–52 | Canis lupus familiaris | Dog | Y | N | NA | NA | NA |
| 121010–53 | Canis lupus familiaris | Dog | Y | Y | Dog | 98.9 | 2.60E-82 |
| 121010–54 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 1.22E-85 |
| 121010–56 | Canis lupus familiaris | Dog | Y | Y | Dog | 98.9 | 7.22E-83 |
| 121110–55 | Canis lupus familiaris | Dog | Y | Y | Dog | 99.4 | 5.54E-84 |
| 170211–12 | Canis lupus familiaris | Dog | N | NA | NA | NA | NA |
| 041110–66 | Dasyurus maculatus | Spotted-tailed quoll | Y | Y | Spotted-tailed quoll | 98.4 | 2.08E-83 |
| 101110–9 | Dasyurus maculatus | Spotted-tailed quoll | Y | Y | Spotted-tailed quoll | 98.2 | 2.33E-72 |
| 170211–25 | Dasyurus maculatus | Spotted-tailed quoll | Y | Y | Spotted-tailed quoll | 99.4 | 1.55E-84 |
| 041110–01 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 99.4 | 2.25E-72 |
| 041110–04 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 2.05E-88 |
| 041110–07 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 4.80E-74 |
| 041110–15 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 1.01E-54 |
| 041110–74 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 1.19E-85 |
| 041110–80 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 9.34E-87 |
| 100111–05 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 3.34E-86 |
| 100111–31 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 3.34E-86 |
| 120111–32 | Dasyurus viverrinus | Eastern quoll | N | NA | NA | NA | NA |
| 120111–33 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 2.61E-87 |
| 170211–14 | Dasyurus viverrinus | Eastern quoll | Y | Y | Eastern quoll | 100 | 2.61E-87 |
| 100111–04 | Felis catus | Feral cat | Y | Y | Feral cat | 100 | 1.54E-79 |
| 120111–10 | Felis catus | Feral cat | Y | Y | Feral cat | 100 | 1.56E-79 |
| 120111–12 | Felis catus | Feral cat | Y | Y | Feral cat | 100 | 1.58E-79 |
| 120111–31 | Felis catus | Feral cat | Y | N | NA | NA | NA |
| 170211–13 | Felis catus | Feral cat | Y | Y | Feral cat | 99.2 | 3.36E-60 |
| 170211–21 | Felis catus | Feral cat | Y | Y | Feral cat | 100 | 1.61E-79 |
| 170211–22 | Felis catus | Feral cat | Y | Y | Feral cat | 100 | 1.55E-79 |
| 041110–42 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 4.02E-80 |
| 041110–47 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 9.34E-87 |
| 041110–48 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 2.61E-87 |
| 041110–53 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 2.47E-82 |
| 041110–59 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 7.32E-88 |
| 121010–06 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 4.02E-80 |
| 121010–22 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 99.4 | 5.58E-84 |
| 200910–24 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 9.34E-87 |
| 200910–25 | Sarcophilus harrisii | Tasmanian devil | Y | Y | Tasmanian devil | 100 | 2.61E-87 |
| 080211–04 | Vulpes vulpes | Red fox | Y | Y | Red fox | 99.4 | 1.22E-85 |
| 080211–05 | Vulpes vulpes | Red fox | Y | Y | Red fox | 99.4 | 5.54E-84 |
| 080211–06 | Vulpes vulpes | Red fox | Y | N | NA | NA | NA |
| 080211–07 | Vulpes vulpes | Red fox | Y | Y | Red fox | 97.2 | 9.35E-61 |
| 080211–08 | Vulpes vulpes | Red fox | Y | Y | Red fox | 99.4 | 5.54E-84 |
| 080211–09 | Vulpes vulpes | Red fox | N | NA | NA | NA | NA |
| 080211–10 | Vulpes vulpes | Red fox | Y | Y | Red fox | 100 | 6.52E-78 |
| 080211–11 | Vulpes vulpes | Red fox | Y | Y | Red fox | 98.9 | 5.66E-84 |
| 080211–12 | Vulpes vulpes | Red fox | Y | N | NA | NA | NA |
| 080211–13 | Vulpes vulpes | Red fox | Y | Y | Red fox | 98.8 | 3.99E-75 |
| 080211–14 | Vulpes vulpes | Red fox | Y | Y | Red fox | 100 | 6.52E-78 |
| 080211–15 | Vulpes vulpes | Red fox | Y | Y | Red fox | 99.1 | 2.63E-50 |
| 080211–16 | Vulpes vulpes | Red fox | Y | Y | Red fox | 100 | 6.52E-78 |
| 080211–17 | Vulpes vulpes | Red fox | N | NA | NA | NA | NA |
| 080211–18 | Vulpes vulpes | Red fox | Y | Y | Red fox | 97.8 | 1.23E-80 |
a% ID is the percentage pairwise identity between the query sequence and the matching sequence identified using BLAST.
bThe e-value represents the number of BLAST hits expected by chance. The lower the e-value is, the better.