Fig. 2.

The effect blood sample size on gametocyte concentration and infectivity by MACS. a The starting material of gametocyte-infected whole blood was 300 µL for the controls, which was fed to mosquitoes directly. For the MACS, starting material was 300, 1500 and 4500 µL, respectively. Gametocytes from this starting material were concentrated, eluted and reconstituted in 400 µL of uninfected blood, from which 300 µL was fed to the mosquitoes. Vertical bars indicate mean with SEM. ***p < 0.001. b Gametocyte density per sample from feeding material measured by Pfs25 qRT-PCR