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Spatial control of EGFP expression through optical regulation of splice-switching using the LASSO. Localized irradiations were performed using a vertical mask (A) or a partially closed microscope shutter (B). Cells were imaged after 24 h. Images of the EGFP channel (left) and the EGFP channel merged with a brightfield image (right) are shown. Scale bars indicate 0.2 mm.
