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. Author manuscript; available in PMC: 2017 Aug 6.
Published in final edited form as: J Am Chem Soc. 2015 Mar 3;137(10):3656–3662. doi: 10.1021/jacs.5b00580

Figure 5.

Figure 5

Optical regulation of splice switching in living zebrafish embryos. Schematic for the RNA splicing (black) and its deactivation by the LASSO. Dark blue boxes represent 2′OMe-NPOM-caged uridine residues. (A) In the absence of irradiation, the sox31 LASSO does not bind to the target site and the sox31 mRNA is correctly spliced, resulting in gene expression. (B) UV activation of the sox31 LASSO enables conditional splice blocking of the sox31 RNA, resulting in inhibition of gene expression. (C) Zebrafish embryos were imaged at 8 hpf, showing the major phenotype observed from treatment with the light-activated and control splice-switching oligonucleotides, both without (top) and with (bottom) UV irradiation. Arrows indicate the absence of epiboly formation. (D) Zebrafish embryo scores were determined for the frequency of no epiboly formation with and without UV exposure (365 nm, 2 min). Error bars represent standard deviations from three independent experiments. N = 18–44.

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