Fig. 2.
Serum BPA-G measurements in vehicle, 2.5, and 25 μg BPA/kg bw/day animals from the BPA core chronic study. Vehicle (continuous and stop dose arms) and BPA-dosed (continuous arm only) males and females were evaluated. At a single time point within one week of termination at 12 months of age, rats from each of the 5 housing rooms of the core chronic study and representing all 5 study loads were sampled from the tail vein to provide approximately 0.5 ml of blood. The blood sample from animals in the continuous dosing arm was drawn 15–60 min after gavage, approximately Cmax following gavage administration [8]. Rats from the vehicle control stop dose arm were sampled at approximately the same time of the day. Serum samples were analyzed by LC–MS/MS blinded to treatment group. The limit of detection (LOD) was established on each day of analysis and the range is indicated by the two horizontal lines on the graph. Red symbols indicate BPA-G measurements that were <LOD and are indicated as 1/2 LOD. X-axis labels, their definitions, and number of animals sampled are as follows: 0 Cont F and 0 Cont M represent continuous dose vehicle control females and males, respectively, n = 13/sex; 0 Stop F and 0 Stop M represent stop dose vehicle control females and males, respectively, n = 10/sex; 2.5 Cont F and 2.5 Cont M represent continuous dose 2.5 μg BPA/kg bw/day females and males, respectively, n = 15/sex; 25 Cont F and 25 Cont M represent continuous dose 25 μg BPA/kg bw/day females and males, respectively, n = 15/sex.