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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2017 May 5;628:71–80. doi: 10.1016/j.abb.2017.05.002

Figure 2. Illustration of the CHESPA approach.

Figure 2

1H-15N resonance assignments for apo, mutant and cAMP-bound forms of EPAC are represented as grey, green, and red circles, respectively. The compounded chemical shift upon mutation and ligand binding is calculated as the magnitude of vectors A and B, respectively (see text for details). θ represents the angle between vector A and B. Figure adapted from ref. 43.