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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2017 Mar 2;628:123–131. doi: 10.1016/j.abb.2017.02.009

Figure 3. 15N incorporation from Glutamine in purine nucleotides.

Figure 3

Spectral editing via 1H{15N}-HSQC detects 15N-isotopomers of nucleotides. A549 cells were grown for 24 h in the presence of 5 mM unlabeled glucose and 4 mM [U–13C,15N]-glutamine. The spectra of the polar extract were recorded at 14.1 T at 20 °C with acquisition times of 0.15 s in t2 and 0.021 s in t1 and a recycle time of 1.6 s. The value of the INEPT transfer delay was set to 10 ms.

A. 1D 1H{15N}-HSQC spectra at different time points. B. 2D HSQC shows editing of the complex proton spectrum. The long range 1H-15N couplings shows utilization of both the amido (blue) and amino N of Gln. Adapted from ref. [2] “With permission of Springer”