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. 2017 Aug 7;8:203. doi: 10.1038/s41467-017-00224-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — l-His inhibition kinetics in the presence of TIH. a Four double-reciprocal plots showing linear uncompetitive inhibition patterns determined for l-His in the presence of 0 (black), 0.25 (red), 0.8 (blue) and 4 (green) mM TIH, at fixed variable concentrations of ATP. Results are representative of two independent experiments. Points are experimental data obtained with 0 (black circles), 12 (white circles), 24 (black squares) and 36 (white squares) µM l-His. Lines are the best fit of each uncompetitive pattern data set to Eq. 5 in Methods section. b Secondary replot of the slopes (circles) and intercepts (squares) of the data shown in a. c Tertiary replots of the slopes (top panel) and intercepts (lower panel) of the data shown in b. Slope of the intercept (triangles), intercept of the intercept (diamonds) and intercept of the slope (hexagons). Lines are the best fit to Eq. 7 in Methods section. d Kinetic model for a combination of uncompetitive inhibition and non-essential activation. The complexes in boxes are in equilibrium. The blue box indicates that this complex is always present in the assay. E, Enzyme; S_A, ATP; S_P, PRPP; A, TIH; I, l-His; P, Product