Table 2.
The percent of T cells and values of IFN-γ, IL-4, TGF-β, FOXP3, and IL-17 gene expressions as well as IFN-γ/IL-4 ratio of splenocyte in asthmatic groups treated with the extract of Z. multiflora at concentrations 200, 400, and 800 μg/ml (groups Z1, Z2, and Z3, respectively) and dexamethasone (D, 0.1 mM).
| Variable | Z1 | Z2 | Z3 | D |
|---|---|---|---|---|
| Th1 | 15.66 ± 0.88*** | 16 ± 1.29*** | 15.66 ± 1.38*** | 5.4 ± 0.54 |
| Th2 | 8.66 ± 0.55 | 7.25 ± 0.25 | 4.5 ± 0.56# | 7.1 ± 2.12 |
| Th17 | 1.1 ± 0.04** | 0.83 ± 0.03 | 0.98 ± 0.05* | 0.6 ± 0.08 |
| Treg | 5.45 ± 0.14** | 7.66 ± 0.25***,### | 5.63 ± 0.22**,††† | 4.08 ± 0.45 |
| Th1/Th2 | 1.85 ± 0.19 | 2.22 ± 0.19 | 3.68 ± 0.49***,##,† | 1.27 ± 0.39 |
| IFN-γ | 323 ± 29.29** | 359.9 ± 45.68*** | 371.5 ± 37.33*** | 112.4 ± 18.71 |
| FOXP3 | 2,196 ± 254.1 | 4,599 ± 228.6***,### | 1,565 ± 262.4††† | 1,580 ± 223.2 |
| IL-4 | 150.7 ± 13.97* | 119.8 ± 20.95 | 102.6 ± 12.18 | 80.62 ± 15.47 |
| TGF-β | 2,667 ± 81.38*** | 2,020 ± 134.6**,# | 1,259 ± 256###,†† | 1,168 ± 8.36 |
| IL-17 | 3,787 ± 89.68 | 6,687 ± 466.7 | 7,496 ± 645.5 | 7,015 ± 618.9 |
| IFN-γ/IL-4 ratio | 2.327 ± 0.42 | 2.91 ± 0.56 | 3.89 ± 0.36** | 1.57 ± 0.25 |
Results are presented as mean ± SEM.
*
p < 0.05,
**
p < 0.01,
***
p < 0.001 vs. dexamethasone (D) group.
#
p < 0.05,
##
p < 0.01,
###
p < 0.001 vs. Z1 group.
†
p < 0.05,
††
p < 0.01,
†††
p < 0.001 vs. Z2 group.
Statistical comparisons were made using ANOVA with Tukey–Kramer multiple post-hoc test.