Figure 6.

Effect of ultrasound stimulus frequency on prostate cancer cell calcium responses. The same set of PC-3 cells (same imaging field of view) was stimulated with different ultrasound transducers at frequencies of 3 MHz [96 V_p–p amplitude, 1 kHz pulse repetition frequency (PRF), 10% duty cycle] and 38 MHz (16 V_p–p amplitude, 1 kHz PRF, 5% duty cycle). The speed of the calcium wave induced at each frequency was 101 µm/s at 3 MHz and 73 µm/s at 38 MHz. Slightly fewer cells responded at 3 MHz than at 38 MHz (278 versus 311).