Skip to main content
. 2017 Aug 7;8:907. doi: 10.3389/fimmu.2017.00907

Figure 1.

Figure 1

Characterization of immune responses to transfused human glycophorin A (hGPA) red blood cells (RBCs), in the presence or absence of poly IC. (A) Serum anti-hGPA IgG at day 5 (D5), day 7 (D7), and day 14 (D14) post-transfusion represented as adjusted mean fluorescence intensity (MFI) in mice transfused with hPGA RBCs in the presence or absence of pretreatment with poly IC. (B) Alloimmunized animals (previously transfused with hGPA RBCs in the presence of poly IC) or non-alloimmunized (previously transfused without poly IC) were transfused for a second time with DiI-labeled syngeneic FVB RBCs and 3,3′-dihexadecyloxacarbocyanine perchlorate (DiO) labeled hGPA RBCs; representative plot showing the gating strategy for DiO and DiO-positive RBCs (pregated on Ter119+ cells). (C) Post-transfusion clearance curve in alloimmunized, non-alloimmunized, or naïve mice. Data are representative of at least two experiments (n = 3–6 mice per group per experiment). **p < 0.01, ****p < 0.0001 determined by Mann–Whitney U test in panel (A) and ANOVA in panel (C), between alloimmunized versus non-alloimmunized or naïve mice. There were no significant differences at any time point between naïve and non-alloimmunized mice.