Figure 2.

Alloantibody formation in the human glycophorin A (hGPA) RBC system is CD4 dependent. (A) Serum anti-hGPA IgG at day 14 represented as adjusted mean fluorescence intensity (MFI) in mice treated with GK1.5 or isotype-matched control antibody during primary transfusion. (B) Serum anti-hGPA IgG after primary or secondary hGPA RBC transfusion, with secondary transfusion given 5 weeks after primary transfusion and 3 weeks after last GK1.5 treatment. (C) Post-transfusion RBC clearance curve in GK1.5-treated, isotype-matched control antibody-treated, or naïve mice after secondary transfusion. *p < 0.05 determined by Mann–Whitney U test. ****p < 0.0001 determined by ANOVA between isotype control or naive and GK1.5-treated mice. There were no significant differences at any time point between naïve and isotype control mice. Data are representative of at least two experiments (n = 3 to 6 mice per group per experiment).