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. 2016 Jul 12;6(7):e852. doi: 10.1038/tp.2016.116

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Copyright © 2016 The Author(s)

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Increased number of agmatinase (Agmat)-expressing cells in male Crtc1^−/− mice. Representative immunofluorescence staining of Agmat-expressing cells (red) and total cells (4,6-diamidino-2-phenylindole (DAPI) staining) in the prefrontal cortex (PFC; a), and in the dentate gyrus (DG; b), CA1 (c) and CA3 (d) regions of the hippocampus (HIP). Cell counting resulted in an increased number of Agmat-expressing cells in the PFC, DG and CA1 regions of male Crtc1^−/− mice (n=6) compared with wild-type (WT) littermates (n=5; e). No difference in number of Agmat-expressing cells was found in the CA3 region. Results are expressed as ratio between number of Agmat-expressing cells and total number of cells. Data are mean ±s.e.m. *P<0.5, **P<0.01. Scale bar, 50 μm.