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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: J Alzheimers Dis. 2013;34(3):727–739. doi: 10.3233/JAD-121669

Fig 3. Glucose and Aβ-mediated apoptosis and tau cleavage in primary cortical neurons.

Fig 3

E15 rat embryonic cortical neurons were cultured for 6 d prior to treatment with glucose, Aβ or both for 24 h. (A) The cell lysates were immunoblotted with the indicated antibodies. The arrowhead indicates the 14 kDa cleaved tau fragment. These experiments were repeated at least 3 times and representative results are shown. (B) Apoptosis was measured by Annexin V staining. *, p<0.05 compared to no treatment. #, p<0.05 compared to 100 mM glucose or 10 µM Aβ treatment. (C) The cortical neurons were grown on PLL-coated glass coverslips before treatment. The cells were fixed with 2% PFA and mounted with ProLong Gold anti-fade mounting media containing DAPI. The arrowheads indicate apoptotic nuclei. The bar represents 15 µm.