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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: J Alzheimers Dis. 2013;34(3):727–739. doi: 10.3233/JAD-121669

Fig 4. Tau cleavage in cortical neurons is prevented by caspase inhibitors.

Fig 4

(A) E15 rat embryonic cortical neurons were treated without (C) or with 100 mM glucose (G), 10 µM Aβ (A) or both (GA) for 24 h in the presence or absence of 20 µM ZVAD. The cell lysates were immunoblotted for the indicated proteins. The asterisk indicates the 14 kDa fragment of tau recognized by the Tau1 antibody. (B) Cortical neurons were treated without or with 100 mM glucose + 10 µM Aβ for 24 h in the presence or absence of 10 nM IGF-I. The cell lysates were immunoblotted for the indicated proteins. The asterisk indicates the 14 kDa fragment of tau recognized by Tau1 antibody. (C) Cortical neuron lysates were treated with glucose and Aβ in the presence of 20 µM ZVAD (Z), calpain inhibitor III (Cl), or lactacystin (L) for 24 h and the cell lysates were immunoblotted with the indicated antibodies. These experiments were repeated at least 3 times and representative results are shown.