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. 2017 Aug 7;8:550. doi: 10.3389/fphys.2017.00550

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Copyright © 2017 Qian, Ni, Niu, Bai, Bao, Li, Sun, Zhang and Teng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR156 and its target PpSPL genes. (A) Alignment of multiple pyr-miR156 target site sequences from PpSPL genes using DNAMAN software. (B) T-plot indicating the cleavage events mediated by miR156ba in PpSPL9 based on degradome sequencing results. (C) Cleavage site mediated by miR156ba in PpSPL9. The cleavage events identified during degradome sequencing were verified by 5′-rapid amplification of cDNA ends (5′-RACE). 17 colonies were sequenced, and 7 of them corresponded to the cleavage site of position 844.