Figure 5.

TK Depletion, let-7a Regulation, and Anti-tumor Effect In Vivo

A total of 1 × 10⁷ RPMI8226-RlucGFP cells were injected subcutaneously into 6-week-old CB.17-SCID mice. Four weeks later, 1 × 10⁶ plaque-forming units of Fluc-expressing viruses (wild-type, ΔTK, and ΔTK let-7a-targeted) were infected intravenously, and the quantity of virus was determined using an in vivo imaging system. (A) Representative in vivo image of tumor mass detected by Renilla luciferase (Rluc). (B) Quantification of Rluc bioluminescence signals. A statistical analysis (n = 10) was performed using Welch’s t test. *p < 0.05; **p < 0.01. Error bars represent SEM.