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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Small. 2017 Feb 17;13(15):10.1002/smll.201603737. doi: 10.1002/smll.201603737

Figure 5. Biomechanical strain induces cytoskeletal reorientation of iPS-SMCs from healthy and HGPS donors.

Figure 5

(A) Schematic of the methodology used to explore biomechanical changes in a context of vascular aging. (B) mRNA expression levels of progerin confirmed its overexpression HGPS cells (bars represent mean ± SD of n=5). F-actin was stained for (C–D) healthy or (E–F) HGPS iPS-SMCs under 0% and 16% strain for 24 hours and the corresponding angle orientation distribution was determined (#, P<0.0001; scale bars represent 50 μm; red arrow indicates direction of strain). (G) HGPS iPS-SMCs showed increase levels of lamin A/C/progerin (antibody against epitope corresponding to aminoacids 231–340). (H) Quantification of lamin A/C/progerin levels.