Figure 1. Fgf10 is essential for SMG epithelial development.
Whole mount immunostaining of an E12 wildtype (Fgf10+/+) SMG and Fgf10−/− SMG mesenchyme. SMG development involves the formation of an epithelial endbud and primary duct (E-Cadherin, green) within a condensed mesenchyme, which is surrounded by an endothelial cell plexus (PCAM, cyan blue) and the formation of a PSG (Tubb3, red). White dotted lines outline PSG, white dashed line is the oral epithelium (oral ep); arrows indicate nerves in the adjacent tongue and oral epithelium. Scale bar, 50 μm. From Figure 2B in Knosp et al., 2015.