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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: J Med Chem. 2006 Dec 28;49(26):7836–7842. doi: 10.1021/jm060803i

Figure 5.

Figure 5

Regulation of G1 phase cell cycle regulators in LNCaP cells. Cell lysates were collected following treatment with vehicle (control) or 50 nM of either 1, 2, 3 or DOX for 72 h. (A) Western blot analysis with anti-cdk4, -cdk6 and -cyclin D1, (B) Western blot analysis with anti- cdk2 and -cyclin E, and cdk2 kinase assay; 32P-HH1, γ-32P-histone H1. (C) Western blot analysis with anti-p21, -p27 and -p53. Actin was used as loading control. RE, relative expression of proteins was determined by densitometry and normalized to actin.