Fig. 3.

Presence of OSCs in aged mouse and human ovaries. (a) Yield of OSCs (as a percent of the total viable cells sorted; see White et al⁹ and Woods and Tilly¹¹ for methodological details) from ovaries of C57BL/6 female mice throughout reproductive life into advanced ages (graph represents results from analysis of a minimum of four pooled ovaries for each OSC sort at each age, with each age point replicated a minimum of three times using different mice for each replicate; mean ± SEM). (b) Yield of OSCs (as a percent of total viable cells sorted) from ovaries of women during reproductive life and through peri- and postmenopausal years (see White et al⁹ and Woods and Tilly¹¹ for methodological details). (c) Example of an immature oocyte formed spontaneously in cultures of OSCs isolated from ovarian cortical tissue of a woman at 53 years of age.