Fig.1.

In vitro testing. EGFR-negative H520 (NucLight red) were mixed with EGFR-positive A549 cells (Fig. 1A). The left panel shows bright field images of all cells, the middle panel shows green fluorescence of the CNR binding the EGFR-positive A549 cells (Donkey anti human Dylight labelled secondary antibody) and the Right panel shows the Red fluorescing H520 cells (location indicated by the blue arrows in each panel). MB49 bladder cancer cells were treated with CNR and laser at increasing exposure duration with cell survival assessed by MTT assay. Standard deviation is shown (Fig. 1B). MB49 cells were incubated with the guiding C-225 antibody and the CNR construct to assess any inherit toxicity from the CNR in the absence of laser. An MTT assay was performed after 5 days (Fig. 1C).