Fig.2.

Establishment of new orthotopic xenograft bladder cancer model. After transfection with Lenti-luc virus, the T24 cells were seeded into 96 well plate and single clones with positive Luciferase activity were selected (Fig. 2A). These cells were cultured and Luciferase activity was further confirmed (Fig. 2B). The cells were implanted intravesically into the mouse bladders and a 90% tumor implantation rate was observed. Mice with positive tumor growth at one week were followed for two months, demonstrating tumor implantation and persistence (Fig. 2C). Representative pictures of mice at 2 days post laser treatment with the laser doses of 3.2 W/cm² for 15 seconds (left), 3.2 W/cm2 for 30 seconds (middle) and 2.1 W/cm² for 30 seconds (right) (Fig. 2D). The mouse in the middle shows slight erythema and skin contraction in contrast to the other mice which show no evidence of skin changes.