Figure 4. Hyperactivation of the PI3K-mTOR Pathway in mat ΔIG Fetal Liver HSPCs.

(A) Poly-A⁺ RNA-seq workflow.

(B) MA plot (plot of mean log₂ difference versus mean log₂ intensity) for differential expression analysis between WT and mat ΔIG fetal liver LSK cells.

(C) GO enrichment analysis using −log₁₀ of the uncorrected p value as x axis. The upregulated or downregulated GO terms in mat ΔIG are shown in red or blue, respectively. The numbers indicate the amount of enriched genes in each term.

(D–G) Representative image and quantification of staining intensity of LSK cells sorted from WT and ΔIG fetal livers for p-Akt^S473 (n ≥ 43) and p-mTOR^S2448 (n ≥ 152).

(H–K) MFIs of p-Akt^S473 and p-mTOR^S2448 in fetal liver HSCs (H and I, n = 4) and donor CD49b^lo HSCs from BM of 3^rd recipients (J and K, n ≥ 4).

Error bars, SEM (H and I) or SD. Scale bars, 5 μm. *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S4, Table S4, and Table S7.