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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: Cell Stem Cell. 2015 Nov 25;18(2):214–228. doi: 10.1016/j.stem.2015.11.001

Figure 6. Loss of Imprinting at the Dlk1-Gtl2 Locus Enhanced Mitochondrial Biogenesis and Metabolic Activity and Increased ROS Levels in HSCs.

Figure 6

(A–D) MFI of PGC-1α and p-4E-BP1T37/46 in fetal liver HSCs (A and B, n = 3) and donor CD49blo HSCs from BM of 3rd recipients (C and D, n ≥ 4).

(E) Mitochondrial mass of WT and mat ΔIG cells assayed by MitoTracker Green staining (n = 3).

(F) Mitochondrial membrane potential of WT and mat ΔIG cells assayed by DilC5 staining (n = 3).

(G) Relative mitochondria DNA copy number of WT and mat ΔIG cells assayed by qRT-PCR (n = 2).

(H and I) Representative TEM images of mitochondrial morphology in WT and mat ΔIG LSK cells.

(J) Glucose uptake of WT and mat ΔIG cells assayed by 2-NBDG (n = 3).

(K) Relative basal ATP levels of WT and mat ΔIG cells (n = 2).

(L) ROS levels of WT and mat ΔIG cells assayed by H2-DCFDA (n = 3).

(M) Percentage of apoptotic cells in WT and mat ΔIG cells assayed by Annexin V and 7-AAD staining (n = 3).

Error bars, SEM or SD (C and D). *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S6 and Table S7.