Table 2.
Optimization of pH, temperature, carbon and nitrogen sources for biosurfactant production by strains Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3: analysis of variance (ANOVA)
| Treatment | Pseudomonas stutzeri NA3 | Acinetobacter baumannii MN3 | ||||
|---|---|---|---|---|---|---|
| df | F | P | df | F | P | |
| pH | ||||||
| 5 | 5 | 13.50 | 0.021 | 5 | 11.786 | 0.026 |
| 6 | 5 | 14.76 | 0.018 | 5 | 9.600 | 0.036 |
| 7 | 5 | 18.69 | 0.012 | 5 | 29.817 | 0.005 |
| 8 | 5 | 9.046 | 0.040 | 5 | 38.020 | 0.004 |
| 9 | 5 | 9.600 | 0.036 | 5 | 9.926 | 0.034 |
| 10 | 5 | 8.824 | 0.041 | 5 | 8.436 | 0.044 |
| Temperature | ||||||
| 20 °C | 5 | 28.680 | 0.006 | 5 | 17.246 | 0.014 |
| 30 °C | 5 | 22.072 | 0.009 | 5 | 11.616 | 0.027 |
| 40 °C | 5 | 25.835 | 0.007 | 5 | 11.906 | 0.026 |
| 50 °C | 5 | 8.824 | 0.041 | 5 | 16.769 | 0.015 |
| 60 °C | 5 | 21.600 | 0.010 | 5 | 8.517 | 0.043 |
| Carbon sources | ||||||
| Crude oil | 5 | 55.846 | 0.002 | 5 | 10.367 | 0.032 |
| Coconut oil | 5 | 11.173 | 0.029 | 5 | 28.628 | 0.006 |
| Diesel oil | 5 | 18.744 | 0.012 | 5 | 15.970 | 0.016 |
| Sucrose | 5 | 8.267 | 0.045 | 5 | 14.107 | 0.020 |
| Starch | 5 | 21.407 | 0.010 | 5 | 11.361 | 0.028 |
| Glycerol | 5 | 10.584 | 0.031 | 5 | 43.333 | 0.003 |
| Mannitol | 5 | 26.427 | 0.007 | 5 | 12.448 | 0.024 |
| Maltose | 5 | 7.983 | 0.048 | 5 | 9.047 | 0.040 |
| Nitrogen sources | ||||||
| Ammonium phosphate | 5 | 51.923 | 0.002 | 5 | 20.450 | 0.011 |
| Ammonium sulphate | 5 | 9.600 | 0.036 | 5 | 5.880 | 0.072 |
| Ammonium chloride | 5 | 18.150 | 0.013 | 5 | 9.750 | 0.035 |
| Peptone | 5 | 33.231 | 0.004 | 5 | 9.720 | 0.036 |
| Potassium nitrate | 5 | 49.148 | 0.002 | 5 | 23.520 | 0.008 |
| Yeast | 5 | 34.645 | 0.004 | 5 | 18.692 | 0.012 |
| Urea | 5 | 22.982 | 0.009 | 5 | 9.213 | 0.039 |
df degrees of freedom, F statistics, P value