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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: Acta Physiol (Oxf). 2015 Jul 19;216(1):132–145. doi: 10.1111/apha.12548

Figure 2.

Figure 2

KCa3.1 regulates cell volume in neutrophils. (a) summarizes cell volume changes in human neutrophils incubated with ionomycin (5 μM) in the presence or absence of TRAM-34. n = 6 and *P < 0.05, **P < 0.01 two-way ANOVA. (b) Images are representative of calcein-loaded neutrophils (white arrowheads) observed at three different time points during the experiments presented in (c) (scale bar indicates 10 μm). (c) corresponds to traces for time course of relative fluorescence for calcein-loaded mouse neutrophils subjected to hypotonic shock (from 300 to 200 mOsm kg−1). Numbers and black arrowheads indicate the time at which pictures showed in (c) were taken. A total of 27 and 19 cells obtained from 6 Kca3.1+/+ and 4 Kca3.1−/− mice, respectively, were analysed from 3 individual experiments for each genotype. *<0.05 unpaired t-test.