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Thiolase–GFP processing assay. The P. pastoris strains, PPY12 (wt or atg8Δ overexpressing thiolase–GFP), were grown in oleate medium to induce peroxisomes and shifted to starvation medium (SD-N) for the indicated times. Proteins were extracted by TCA precipitation, resolved by SDS-PAGE, and detected with anti-GFP antibody. The figure was kindly provided by Dr. Jean-Claude Farré.
