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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: Methods Enzymol. 2016 Nov 30;588:413–427. doi: 10.1016/bs.mie.2016.09.088
Strains
Name
Description Genotype Source
PPY12 wt P. pastoris strain his4 arg4 Gould, McCollum, Spong, Heyman, and Subramani (1992)
BFP–SKL Overexpression of BFP–SKL in PPY12 PPY12 PrGAPDH-BFP–SKL::ARG4 his4 arg4 Farre et al. (2008)
atg30Δ atg30 deletion in BFP–SKL-expressing strain PPY12 atg30Δ::KanMX, PrGAPDH-BFP–SKL::ARG4 his4 arg4 Farre et al. (2008)
atg30Δ+Atg30 (wt) Reexpress Atg30 (wt) in atg30Δ strain PPY12 atg30Δ::KanMX, PrATG30-Atg30(wt)-HA::HIS4, PrGAPDH-BFP–SKL::ARG4 his4 arg4 Farre et al. (2008)
atg30Δ+Atg30 (S112A) Reexpress Atg30 (S112A) mutant in atg30Δ strain PPY12 atg30Δ::KanMX, PrATG30-Atg30(S112A)-HA::HIS4, PrGAPDH-BFP–SKL::ARG4 his4 arg4 Farre et al. (2013)