Figure 5.

Bartter mutant degradation is proteasome-dependent in yeast. A–E, cycloheximide chase reactions were performed, and lysates were blotted with anti-HA antibody to measure the stability of ROMK and the indicated mutant proteins over 90 min. In all cases, a pdr5Δ yeast strain was pretreated for 30 min at 37 ºC with 100 μm MG132 (closed squares) or DMSO (open squares). Data represent the means of six independent experiments ± S.E. (error bars). In each experiment, a representative Western blot is shown at the bottom. G6PD was used as a loading control. *, p < 0.05; **, p < 0.01; ***, p < 0.001.