Figure 7.

LPS treatment induces a 20-fold increase in the GroPIns levels in human monocytes. Human peripheral blood monocytes purified from nine donors were treated with LPS (0.1 μg/ml) for 45 min or left untreated as a control, and the intracellular GroPIns was collected by acidified Bligh and Dyer (56) extraction (54) following the modifications reported previously (46). The quantification of the intracellular GroPIns was performed by an LC-MS/MS method recently reported in detail (46). Dots represent single donors, and the horizontal lines indicate the mean values. Statistical analysis was performed using Student's t test (***, p < 0.001).