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. 2017 May 30;292(31):12860–12873. doi: 10.1074/jbc.M117.785030

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — A, Ser(P)²⁴¹-PDK1 assessed by Western blotting in liver extracts from WT and HCV-tg mice injected with insulin or vehicle. The results were normalized using total PDK1 expression (representative animals). B, quantitative results for Ser(P)²⁴¹-PDK1 protein levels. C, Thr(P)³⁰⁸-Akt assessed by Western blotting in liver extracts from WT and HCV-tg mice injected with insulin or vehicle. The results were normalized using total Akt expression (representative animals). D, quantitative results for Thr(P)³⁰⁸-Akt protein levels. E, quantitative results for Ser(P)⁴⁷³-Akt protein levels. F, analysis of Akt activity by kinase assay in hepatic lysates from WT and HCV-tg animals. Akt activity was normalized to the relative expression of Akt in the lysates and is expressed as the percentage of WT Akt activity.