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. 2017 Jun 15;292(31):12874–12884. doi: 10.1074/jbc.M116.769943

Figure 3.

Figure 3.

USP9X is a centrosomal protein and functions to regulate the abundance of PCM1 and CEP55 in centrosome. A, U2OS cells were fixed and stained with antibodies against USP9X and Centrin or γ-tubulin. Scale bar, 10 μm. B, U2OS cells transfected with control siRNA or USP9X siRNA were fixed and subjected to immunostaining with antibodies against PCM1 and USP9X. The percentage of cells with USP9X knockdown was counted, and the relative intensity of PCM1 or USP9X in the centrosome was analyzed by ImageJ software. Error bars represent S.D. for biological triplicate experiments. **, p < 0.01, Student's t test. Scale bar, 10 μm. C, U2OS cells transfected with control siRNA or USP9X siRNA were fixed and subjected to immunostaining with antibodies against CEP55 and USP9X. The percentage of cells with USP9X knockdown was counted, and the relative intensity of CEP55 or USP9X in the centrosome was analyzed by ImageJ software. Error bars represent S.D. for biological triplicate experiments. **, p < 0.01, Student's t test. Scale bar, 10 μm. D, U2OS cells transfected with control siRNA or USP9X siRNA were fixed and subjected to immunostaining with antibodies against Pericentrin and USP9X. The percentage of cells with USP9X knockdown was counted, and the relative intensity of Pericentrin or USP9X in the centrosome was analyzed by ImageJ software. Error bars represent S.D. for biological triplicate experiments. **, p < 0.01, Student's t test. Scale bar, 10 μm.