Figure 6.

USP9X promotes centrosome biogenesis. A, control U2OS cells or U2OS cells stably expressing USP9X-WT, catalytic mutant USP9X (USP9X-CS), or USP9X-ΔN were transfected with control siRNA or USP9X 3′-UTR siRNA for 96 h followed by hydroxyurea treatment. Then the cells were fixed and immunostained with antibodies against the indicated proteins. Scale bar, 10 μm. B, population of cells from A with the indicated numbers of Centrin foci was counted. Error bars represent S.D. for biological triplicate experiments. **, p < 0.01, one-way ANOVA. C, cellular extracts from A were prepared and analyzed by Western blotting with antibodies against the indicated proteins.