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Proposed chemical mechanism of covalent flavinylation via a quinone-methide intermediate. Catalytic bases are shown in blue. The identities of the residues involved in proton abstraction and intermediate stabilization in QFR have not been unambiguously determined, but likely possibilities are shown here. The FrdAH44 side chain (red) is the nucleophile that forms a covalent bond, and may also be the base for proton abstraction.
