. 2017 Jun 5;292(31):12947–12958. doi: 10.1074/jbc.M116.760199

Table 2.

Channel properties of RyR1-WT and mutant channels

	WT-RyR1	Q4933A	Q4933V	G4934A	G4934V	I4937A	I4937V	I4937T
Immunoblot intensity (% WT)	100	50.3 ± 15.5(6)^a	42.1 ± 9.6(6)^a	79.5 ± 19.5(7)	79.6 ± 17.1(6)	35.1 ± 17.7(6)^a	55.3 ± 14.6(6)^a	69.9 ± 16.5(6)
Caffeine response (% WT)	100	76.4 ± 11.7(5)	24.4 ± 10.8(5)^a	171.5 ± 16.7(4)^a	78.7 ± 23.2(6)	103.8 ± 25.1(5)	168.4 ± 35.7(5)	124.2 ± 12.3(5)
[³H]Ryanodine binding (% WT)	100	0.5 ± 0.4(7)^a	1.9 ± 1.8(5)^a	70.0 ± 10.7(4)^a	11.5 ± 1.8(5)^a	3.2 ± 2.8(7)^a	71.4 ± 8.8(5)^a	27.7 ± 2.0(6)^a
P_o (2 μm Ca²⁺)	0.11 ± 0.03(13)	0.17 ± 0.07(14)	0.59 ± 0.22(4)^a	0.05 ± 0.01(9)	0.10 ± 0.03(4)	0.27 ± 0.11((7)	0.44 ± 0.08(7)^a	0.15 ± 0.05(9)
P_o (0.1 μm Ca²⁺)	0.01 ± 0.01(9)	0.01 ± 0.01(9)	0.57 ± 0.23(4)^a	0.01 ± 0.01(9)	0.10 ± 0.03(4)^a	0.34 ± 0.18(4)	0.20 ± 0.08(7)^a	0.05 ± 0.04(3)
γ_K+ (pS)	766 ± 11(12)	762 ± 9(14)	693 ± 50(4)	635 ± 26(9)^a	539 ± 138(4)^a	597 ± 79(7)	793 ± 21(7)	742 ± 14(7)
I_Ca (pA) (10 mm trans Ca²⁺)	−2.3 ± 0.1(9)	−1.6 ± 0.1(7)^a	−0.5 ± 0.2(4)	−1.5 ± 0.1(9)^a	−1.1 ± 0.2(4)^a	−0.1 ± 0.1(4)	−2.0 ± 0.1(7)^a	−1.7 ± 0.2(5)^a
P_Ca/P_K	6.6 ± 0.2(8)	3.3 ± 0.2(7)^a	0.3 ± 0.1(4)^a	5.0 ± 0.2(9)^a	3.6 ± 0.6(4)^a	ND^b	6.5 ± 0.1(7)	4.7 ± 0.4(5)^a
No. of events/min	8125 ± 1086(11)	2899 ± 937(5)^a	11,154 ± 5729(5)	2379 ± 586(8)^a	930 ± 261(4)^a	ND	7259 ± 2236(4)	8000 ± 2203(6)
T_o (ms)	0.71 ± 0.14(11)	1.57 ± 1.20(5)	25.2 ± 23.2(5)	0.64 ± 0.08(8)	8.56 ± 3.74(4)^a	ND	4.26 ± 1.59(4)^a	0.70 ± 0.17(6)
T_c (ms)	11.0 ± 3.5(11)	57.9 ± 37.0(5)^a	32.8 ± 31.3(5)	38.7 ± 9.5(8)^a	78.1 ± 22.3(4)^a	ND	5.78 ± 2.09(4)	18.5 ± 11.0(6)

^a p < 0.05 compared to WT-RyR1.

^b ND, not determined. Kinetic parameters of channel gating were obtained from recordings that contained a single channel in bilayers.