Loss of binding ability for exogenous all-trans-retinal in the Platynereis c-opsin K94T mutant.
A–D, absorption spectra of Platynereis c-opsin K94T mutant samples that were purified after incubation with 11-cis (A), all-trans (B), 13-cis (C), or 9-cis (D) retinal. The λmax values after incubation with 11-cis or 9-cis retinal are indicated. Note that, after incubation with all-trans or 13-cis retinal, no absorbance except protein absorbance at ∼280 nm was observed.