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. 2017 Jun 20;292(31):13022–13033. doi: 10.1074/jbc.M117.795682

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — The MftA M1W/V29A mutant led to the accumulation of MftA M1W/V29A** product. HPLC analysis of the reactions shows the conversion of MftA M1W/V29A (black) to MftA* (13.7 min) and MftA** (14.1 min). Analyses were carried out on a C4 HPLC column. B, the absorbance spectra of MftA M1W/V29A (black), MftA M1W/V29A* (red), and MftA M1W/V29A** (blue) were similar to what was found for reactions with MftA M1W. C, ¹H NMR analysis of MftA M1W/V29A** demonstrated the formation of an αβ-unsaturated indicated by appearance of doublets at ∼6.3 and ∼7.2 ppm (indicated by **). mAu, milliabsorbance units.