Figure 1.

Determination of site size of mtSSB tetramer on poly(dT) DNA. A, titrations of the mtSSB tetramer (40 nm) with increasing concentrations of poly(dT) were performed by monitoring protein tryptophan fluorescence quenching in buffer A with 20 mm NaCl (black), 100 mm NaCl (red), or 500 mm NaCl (blue), in the presence of 10 mm MgCl₂. The site sizes were determined by fitting the data to Equation 5. Fitted curves are superimposed on the data. B, the derived site size is plotted against the [NaCl]. Error bars, S.D.