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Relative volumes of nuclei and nucleoli increase with DNA Index R. (A) 3-dimensional surface rendering of deconvolved z-stacks of representative ploidy-sorted nuclei. For each sample, 10–40 nuclei were assessed. Scale bar: 5 µm. (B) Nuclear and nucleolar volumes (µm3) were, respectively, normalized to those ones of the 4E, and represented in function of DNA Index R. Nucleolus from 2C-nuclei was too small and undetectable for volume estimation.
