Skip to main content
. Author manuscript; available in PMC: 2018 Jul 24.
Published in final edited form as: Vaccine. 2017 Jun 29;35(33):4287–4294. doi: 10.1016/j.vaccine.2017.04.052

Figure 1. Expression of zEDIII in E. coli.

Figure 1

(A) zEDIII expression is driven by the T7 promoter (PT7) under the control of the lac repressor. A ribosomal binding site (RBS) is provided upstream of the zEDIII coding sequence to enhance its translational efficiency. The coding sequence of zEDIII is fused to an 18-bp sequence that codes for a hexa-histidine tag (His6) at its N-terminus for efficient purification and detection of the target protein. (B) Samples of the E. coli BL-21 culture were collected at various time points after induction with IPTG and total cellular proteins were analyzed by a 15% SDS-PAGE under reducing condition, followed by Coomassie blue staining. Lane 1, Total protein sample from non-induced E. coli as a negative control; Lanes 2 and 3, total protein samples from E. coli collected 4 and 12 hr post IPTG induction.