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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 May 8;108:918–928. doi: 10.1016/j.freeradbiomed.2017.05.009

Fig. 2. The effects of Nrf2 knockdown in KC on UVB-induced apoptosis of MC.

Fig. 2

Dose-dependent effects of UVB on annexin V positivity (A) and active caspase-3 (B) in MC alone. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. *P < 0.05; **P < 0.01; ***P < 0.001 versus unirradiated MC monoculture. (C) Dose-dependent effects of UVB on active caspase-3 in MC pretreated with CM from KC irradiated with UVB for 30 min. The statistical significance of differences between unirradiated MC and UVB-irradiated MC monoculture was evaluated by one-way ANOVA followed by Dunnett’s test (**P < 0.01; ***P < 0.001 versus unirradiated control MC) and between the UVB-irradiated MC monoculture and UVB-irradiated MC+KC-CM was evaluated by Student’s t-test (#P < 0.05; ##P < 0.01 versus UVB-irradiated MC monoculture). The effects of UVB (250 mJ/cm2) on annexin V positivity (D) and active caspase-3 (E) in MC monoculture, MC co-cultured in transwell with siNrf2 or siCtrl-transfected KC and MC pretreated with the CM from siNrf2 or siCtrl-transfected KC following UVB irradiation. MC were harvested at 12 h after UVB irradiation for determination of annexin V/PI and active caspase-3 staining. Data were expressed as mean ± SD. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. **P < 0.01, ***P < 0.001 versus UVB-irradiated MC monoculture. #P < 0.05; ###P < 0.001 versus UVB-irradiated MC+KC (siNrf2) or UVB-irradiated MC+KC (siNrf2)-CM.