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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2017 May 8;108:918–928. doi: 10.1016/j.freeradbiomed.2017.05.009

Fig. 7. Involvement of Nrf2 in the modulation of paracrine effect of KC on inflammatory response is ROS-dependent.

Fig. 7

The effects of UVB (125 mJ/cm2) on nuclear translocation of p65 (A) and p50 (B) NF-κB subunits and TNF-α release (C) in MC incubated with CM from Nrf2-depleted KC pretreated with NAC. Western blotting was performed to determine nuclear tranlocation of p65 and p50 NF-κB subunits at 6 h following UVB irradiation. The p65 and p50 NF-κB subunits were detected at 65 and 50 kDa, respectively, lamin A, the loading control for nuclear protein, at 69 kDa and β-actin, the loading control for cytosol protein, at 42 kDa. TNF-α levels were quantified at 24 h post-irradiation using an ELISA kit. Data were expressed as mean ± SD. The statistical significance of differences was evaluated by Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001 versus irradiated MC+KC (siCtrl)-CM. #P < 0.05 ## P < 0.01 versus UVB-irradiated MC+KC (siNrf2)-CM without NAC pretreatment.