Figure 4.

Quantification of proliferation in response to cdk-6 and cyclin D₁ . Dissociated islet cells infected for 24 h with increasing total multiplicity of infection (MOI) of cdk6 and cyclin D₁ and incubated for 3 days were imaged and quantified for cell identity and proliferation status. (A) The percentage of proliferating C-peptide-expressing beta cells was quantified in each well of a 96-well plate. (B) The percentage of proliferating C-peptide-negative cells (including other islet endocrine cell types, endothelial cells, and fibroblasts) was quantified in each well of a 96-well plate. (C) Islet cells were infected with cdk-6 and cyclin D₁ at an MOI of 200 for each gene in the absence or presence of indicated concentrations of the immunosuppressants rapamycin, FK506, or both. After a 3-day incubation, cells were fixed, stained, and quantified in the same manner as above. Data represent the mean ± standard deviations of 32 independent wells.