Figure 1.

Constitutive deletion of the VGAT from AgRP cells does not affect the frequency of spontaneous IPSCs recorded from POMC cells despite abolishing evoked IPSCs between these cells. A, sIPSCs recorded from POMC cells with intact VGAT in AgRP neurons (top) and after deletion of VGAT from AgRP cells (bottom). All voltage-clamp experiments were conducted at a holding potential of −60 mV. B, Summary figure comparing the frequency of sIPSCs recorded from POMC cells in mice with intact GABA release from AgRP neurons and mice where GABA release has been removed from AgRP neurons by deleting VGAT in these cells (AgRP-VGAT^WT, n = 17; AgRP-VGAT^KO, n = 14; p = 0.917, unpaired t test). Data are presented as the mean ± SEM. C, Twenty consecutive traces demonstrating the reliability of light-evoked GABA release from AgRP neurons onto POMC neurons (top trace) and the ablation of this connection following deletion of VGAT from AgRP cells (bottom trace). A 2 ms flash of 470 nm light is indicated by a blue dash above traces.