Figure 2.

Increased frequency of sIPSCs in POMC cells following overnight fast is not attenuated by deletion of VGAT in AgRP cells. A, Representative traces showing that an overnight fast increases sIPSC frequency in POMC cells in recordings from both mice with intact VGAT in AgRP neurons (AgRP-VGAT^WT) and in mice where VGAT was deleted from AgRP neurons (AgRP-VGAT^KO). B, Overnight fast increases spontaneous GABA release onto POMC cells in hypothalamic slices obtained from animals with intact VGAT in AgRP cells (fed, n = 17; fasted, n = 15; *p = 0.04, unpaired t test). C, In hypothalamic slices obtained from mice with deleted VGAT in AgRP cells, the fasting-induced increase in spontaneous GABA release onto POMC cells persists (fed, n = 14; fasted, n = 10; *p = 0.02, unpaired t test). All data are presented as the mean ± SEM.