Figure 3.

Deletion of MORs from AgRP neurons does not attenuate the inhibition of sIPSCs in POMC neurons produced by MOR activation. A, Ten to 20 consecutive traces averaged to demonstrate the inhibition of light-evoked IPSCs caused by the MOR agonist DAMGO (10 μm) in recordings from POMC neurons in mice with intact MORs in AgRP cells (AgRP-MOR^WT; left traces) and the block of this effect in mice where MORs were deleted form AgRP cells (AgRP-MOR^KO; right traces). Blue bar above traces indicates a 2 ms pulse of 470 nm light. B, Comparison of the effect of 10 μm DAMGO on light-evoked IPSCs onto POMC cells from AgRP-MOR^WT and AgRP-MOR^KO mice. C, Traces representative of the effect of DAMGO (10 μm) on the frequency of sIPSCs recorded from POMC cells in AgRP-MOR^WT and AgRP-MOR^KO mice. D, Compiled data showing the frequency of sIPSCs recorded from POMC cells at baseline and after bath perfusion of DAMGO (10 μm) from mice with intact MORs on AgRP neurons (***p = 0.00003, paired t test), E, Compiled data showing the frequency of sIPSCs recorded from POMC neurons at baseline and following bath perfusion of DAMGO (10 μm) from mice where MORs were deleted from AgRP neurons (***p = 0.001; paired t test). All data are presented as the mean ± SEM.