The expression of hepatic PPARα protein (A and B), AMPKα and pT172-AMPKα (A and C), and FASN (A and D) were determined by immunoblotting on the left and band quantification was shown on the right. The activation of AMPKα (pT172-AMPKα /AMPKα) was reduced in the BCO2 KO mice compared to WT mice. Values are means ± SD, n=6 mice/group. * Different from WT, p < 0.05. KO, knockout; WT wild type. PPARα, PPAR, peroxisome proliferator-activated receptor α; AMPKα, AMP-activated protein kinase α; FASN, fatty acid synthase